Fabrice Gielen (0000-0003-0604-7224) - ORCID

  • Tiwari, A.;  Daniels, A.M. ;Manley, R. ;  Gielen, F.*, ‘Harnessing droplet microfluidics and morphology-based deep learning for the label-free study of polymicrobial-phage interactions.’’  2025, Communications Biology,

  • Tiwari, A.;  Nikolic, N.;  Anagnostidis, V.; Gielen, F.*, Label-free analysis of bacterial growth and lysis at the single-cell level using droplet microfluidics and object detection-oriented deep learning. Frontiers in Lab on a Chip Technologies 2023, 2.

  • Anagnostidis, V.;  Tiwari, A.; Gielen, F.*, Deep learning-assisted concentration gradient generation for the study of 3D cell cultures in hydrogel beads of varying stiffness. Front Bioeng Biotechnol 2024, 12, 1364553.

  • Ribeiro, A.;  Perez-Arnaiz, P.;  Sanchez-Costa, M.;  Perez, L.;  Almendros, M.;  van Vliet, L.;  Gielen, F.;  Lim, J.;  Charnock, S.;  Hollfelder, F.;  Gonzalez-Pastor, J. E.;  Berenguer, J.; Hidalgo, A., Thermostable in vitro transcription-translation compatible with microfluidic droplets. Microb Cell Fact 2024, 23 (1), 169.

  • Nikolic, N., Anagnostidis, V., Tiwari, A., Chait, R. & Gielen, F. *, Droplet-based methodology for investigating bacterial population dynamics in response to phage exposure, Frontiers in Microbiology 14, 2023.

  • Rogers, S.;  Zhang, C.;  Anagnostidis, V.;  Liddle, C.;  Fishel, M. L.;  Gielen, F.; Scholpp, S., Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2. Proc Natl Acad Sci U S A 2023, 120 (39), e2217612120.

  • Neun, S.;  van Vliet, L.;  Hollfelder, F.; Gielen, F. *, High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform. Anal. Chem. 2022(IF= 6.3).

  • Elvira, K. S.;  Gielen, F.;  Tsai, S. S. H.; Nightingale, A. M., Materials and methods for droplet microfluidic device fabrication. Lab Chip 2022, 22 (5), 859-875.

  • Collins, S., Van Vliet, L., Gielen, F., Janeček, M., Valladolid, S.W., Poudel, C., Fusco, G., De Simone, A., Michel, C., Kaminski, C.F. and Spring, D.R., (2022) A unified in vitro to in vivo fluorescence lifetime screening platform yields amyloid β aggregation inhibitors. bioRxiv, pp.2022-03.

  • Bentley, S. A.;  Anagnostidis, V.;  Schlogelhofer, H. L.;  Gielen, F.* ; Wan, K. Y., Phenotyping single-cell motility in microfluidic confinement. eLife2021 (IF=8.7).

  • Howell, L.;  Anagnostidis, V.; Gielen, F.*, Multi‐Object Detector YOLOv4‐Tiny Enables High‐Throughput Combinatorial and Spatially‐Resolved Sorting of Cells in Microdroplets. Advanced Materials Technologies 2021.

  • Lindenburg, L. H.;  Pantelejevs, T.;  Gielen, F.;  Zuazua-Villar, P.;  Butz, M.;  Rees, E.;  Kaminski, C. F.;  Downs, J. A.;  Hyvönen, M.; Hollfelder, F., Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats, PNAS, 2021.

  • Nikiforov, P. O.;  Hejja, B.;  Chahwan, R.;  Soeller, C.;  Gielen, F.; Chimerel, C., Functional Phenotype Flow Cytometry: On Chip Sorting of Individual Cells According to Responses to Stimuli. Adv Biol (Weinh) 2021, e2100220.

  • Anagnostidis, V., Sherlock, B., Metz, J., Mair, P., Hollfelder, F., Gielen*, F., Deep learning guided image-based droplet sorting for on-demand selection. and analysis of single cells and 3D cell cultures.  Lab Chip., 2020, (doi: 10.1039/d0lc00055h, also deposited on Arxiv:1912.05490, * corresponding author)

  • Buryska, T., Vasina, M., Gielen, F., Vanacek, P., van Vliet, L., Jezek, J., Pilat, Z., Zemanek,     P., Damborsky, J., Hollfelder, F., Prokop, Z., Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics, ANALYTICAL CHEMISTRY. 2019, 91. 10008-10015.

  • Kleine-Bruggeney, H., van Vliet, L. D., Mulas, C., Gielen, F., Agley, C. C., Silva, J. C. R., Smith, A., Chalut, K., Hollfelder, F., Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation,Small. 2019

  • Mair, P., Gielen, F., Hollfelder, F., Exploring sequence space in search of functional enzymes using microfluidic droplets, Curr. Opin. Chem. Biol. 2017, 37. 137-144.

  • Gielen F., M. B., Eric J. Rees, Miklos Erdelyi, Tommaso Moschetti, Marko Hyvonen, Joshua B. Edel, Clemens F. Kaminski, Florian Hollfelder, Fluorescence anisotropy measurements of individual nanolitre droplets enable quantitative steady-state affinity determination. ANALYTICAL CHEMISTRY, 2017 89 (2), 1092-1101.

  • Gielen F., R. H., Stephane Emond, Martin Fischlechner, Ursula Schell, Florian Hollfelder, Ultrahigh Throughput Directed Evolution of Amino Acid Dehydrogenases by Absorbance-Activated Microdroplet Sorting. PNAS, 2016, 113, E7383-E7389.

  • Gielen, F.; Buryska, T.; Van Vliet, L.; Butz, M.; Damborsky, J.; Prokop, Z.; Hollfelder, F., Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets. Analytical Chemistry 2015, 87 (1), 624-632.

  • Colin PY, Kintses B., Gielen F., Miton C., Mohamed M., Morgavi DP., Janssen DB. and Hollfelder F., ‘Functional Metagenomics in Picolitre Droplets for Ultrahigh-throughput Enzyme Discovery’, Nature Communications, 2015, 6.

  • Chen, W. Y.; Avezov, E.; Schlachter, S. C.; Gielen, F.; Laine, R. F.; Harding, H. P.; Hollfelder, F.; Ron, D.; Kaminski, C. F., A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth. Biophysical Journal 2015, 108 (5), 999-1002.

  • Gielen, F.; van Vliet, L.; Koprowski, B. T.; Devenish, S. R. A.; Fischlechner, M.; Edel, J. B.; Niu, X. Z.; deMello, A. J.; Hollfelder, F., A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition. Analytical Chemistry 2013, 85 (9), 4761-4769.

  • Hassan, S. U.; Gielen, F.; Niu, X. Z.; Edel, J. B., Controlled one dimensional oscillation of the Belousov-Zhabotinsky reaction confined within microchannels. Rsc Advances 2012, 2 (16), 6408-6410.

  • Gielen, F.; Demello, A. J.; Edel, J. B., Dielectric Cell Response in Highly Conductive Buffers. Analytical Chemistry 2012, 84 (4), 1849-1853.

  • Niu, X. Z.; Gielen, F.; Edel, J. B.; deMello, A. J., A Microdroplet Dilutor for High-Throughput Screening. Nature Chemistry 2011, 3 (6), 437-442.

  • Gielen, F.; Pereira, F.; deMello, A. J.; Edel, J. B., High-Resolution Local Imaging of Temperature in Dielectrophoretic Platforms. Analytical Chemistry 2010, 82 (17), 7509-7514.

  • Niu, X. Z.; Gielen, F.; deMello, A. J.; Edel, J. B., Electro-Coalescence of Digitally Controlled Droplets. Analytical Chemistry 2009, 81 (17), 7321-7325.

  • Gielen, F.; deMello, A. J.; Cass, T.; Edel, J. B., Increasing the Trapping Efficiency of Particles in Microfluidic Planar Platforms by Means of Negative Dielectrophoresis. Journal of Physical Chemistry B 2009, 113 (5), 1493-1500.

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  • Fabrice Gielen, Pierre-Yves Colin, Philip Mair and Florian Hollfelder, ‘Ultrahigh-throughput screening of single-cell lysates for directed evolution and functional metagenomics’, Protein Engineering – Methods and Protocols, Humana press, 2018

  • Fabrice Gielen, ‘Single cell micro-encapsulation for evolution and discovery of biocatalysts’, “Handbook of Single Cell Technologies”, Springer 2021, (ISBN: 978-981-10-8952-7).